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<\/p>\nSuperox-D has been tested for its ability of being up taken and able to provide protection at intestinal level. A human intestine model of enterocyte-like cell Caco-2 was selected been widely used as a model of the intestinal epithelial barrier. It has been well documented that Caco-2 monolayers represent a reliable correlate for studies on the absorption of drugs and other compounds after oral intake in humans.<\/p>\n
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To assess the ability of being efficiently absorbed in the intestine, the Caco-2 cell model were incubated for 2 hours with media containing different concentration of Superox-D. The ability of Superox-D of being up taken was assessed measuring the Total Antioxidant Activity (TAA) of cell cytosol. The data reported in Figure show that Superox-D is able to effectively penetrate the cell membrane and to induce an increased antioxidant capacity in cell cytosol. This effect is due to the ability of Superox-D of being promptly up taken by the intestine proving the high bioavailability of the product.<\/p>\n
![\"\"](\"https:\/\/superoxide-dismutase.eu\/wp-content\/uploads\/2018\/12\/In-vitro-studies2-300x158.png\")
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The protective effect of Superox-D was assessed by measuring the ability of some enterocyte-like cell Caco-2 model culture of better resisting to intense antioxidant stress. The cell lines were pre treated for 2 hours with media containing different concentration of Superox-D. After the incubation with Superox-D a fresh control medium was used in all samples and the effect of an oxidative stress caused by tert<\/i>-butyl hydroperoxide (t<\/i>-BOOH) was assessed with a fluorometric assessment. As shown in Figure the cell lines pre treated with Superox-D proved to be far more resistant to the radical stress induced during the assay with a dose dependent behavior. Superox-D is therefore able of keeping the intestine protected from the harmful radical species.<\/p>\n
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<\/p>\n","protected":false},"excerpt":{"rendered":"
Superox-D has been tested for its ability of being up taken and able to provide protection at intestinal level. A human intestine model of enterocyte-like cell Caco-2 was selected been widely used as a model of the intestinal epithelial barrier. It has been well documented that Caco-2 monolayers represent a reliable correlate for studies on […]<\/p>\n","protected":false},"author":1,"featured_media":1504,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[13],"tags":[39],"class_list":["post-21","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-sod","tag-superoxide-dismutases"],"yoast_head":"\n